EFFECT OF PRECONDITIONING WITH PHOTOBIOMODULATION ON THE EXPRESSION OF MICRORNAS RELATED TO CARDIAC HYPERTROPHY IN HUMAN VENTRICULAR CARDIOMYOCYTES DERIVED FROM PLURIPOTENT STEM CELLS SUBJECTED TO DOXORUBICIN.

Bruna Calixto de Jesus.; Allan Luís Barboza Atum; Guilherme Rabelo Nasuk; Antônio Henrique Martins; José Antônio Silva Junior

Título del Trabajo

Autores

Bruna Calixto de Jesus.
Allan Luís Barboza Atum
Guilherme Rabelo Nasuk
Antônio Henrique Martins
José Antônio Silva Junior

Modalidad

Investigación

Área temática

Medicina

Fecha de publicación

14/08/2026

País de publicación

Argentina | Argentina

Idioma de la Publicación

es-419

Página del Trabajo

https://www.even3.com.br/anais/ii-encuentro-biofotonica-597461/1253617-effect-of-preconditioning-with-photobiomodulation-on-the-expression-of-micrornas-related-to-cardiac-hypertrophy-

ISBN

978-65-272-2678-9

Palabras Clave

Photobiomodulation, ventricular cardiomyocytes, pluripotent stem cells, miRNAs, cardiotoxicity.

Resumen

INTRODUCTION: Cardiotoxicity is a well-recognized adverse effect associated with cancer therapy, with long-term consequences. Doxorubicin, a chemotherapeutic agent in the treatment of malignant neoplasms, presents as major side effects the cardiac toxicity, raising significant concerns about its clinical use. Photobiomodulation triggers a cascade of molecular events with therapeutic potential in cardiovascular diseases, and the use of light irradiation has been explored as a treatment source to promote beneficial effects in biological tissues. OBJECTIVE: The aim of this study was to analyze the microRNA (miRNA) expression profile in ventricular cardiomyocytes derived from human induced pluripotent stem cells (HiPSC-vCMs) subjected to photobiomodulation preconditioning and exposed to doxorubicin. METHODS: The in vitro experimental study was conducted using HiPSC-vCMs preconditioned with photobiomodulation and exposed to doxorubicin. The HiPSC-vCMs were allocated into three groups: 1 - Control (not exposed to DOX or PBM; irradiated with standard lamp; addition of saline); 2 - DOX group (addition of 2 μM DOX for 24 hours); and 3 - DOX + PBM group (preconditioning with PBM; addition of 2 μM DOX). Dosimetric parameters of the LED device were wavelength of 660 nm ± 10 nm, irradiance of 10 mW/cm², for 500 seconds, resulting in a dose of 5 J/cm². Cytotoxicity analysis was assessed by MTT assay. The expression of microRNAs associated with cardiac hypertrophy was assessed by quantitative real-time polymerase chain reaction (qPCR). RESULTS: Doxorubicin treatment reduced the expressions of miR-1 and miR-208, while increased miR-21 expression. In the DOX+PBM group, the expression levels of these miRNAs were reverted to values similar to the control group. CONCLUSION: Photobiomodulation preconditioning significantly restored the expression of deleterious miRNAs related to cardiac hypertrophy in cardiomyocytes in vitro.

Título del Evento: II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Ciudad del Evento: Buenos Aires
Título de las Actas del Evento: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Nombre de la Editora: Even3
Medio de Divulgación: Meio Digital

Como citar

JESUS., Bruna Calixto de et al.. EFFECT OF PRECONDITIONING WITH PHOTOBIOMODULATION ON THE EXPRESSION OF MICRORNAS RELATED TO CARDIAC HYPERTROPHY IN HUMAN VENTRICULAR CARDIOMYOCYTES DERIVED FROM PLURIPOTENT STEM CELLS SUBJECTED TO DOXORUBICIN... In: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud. Anais...Sao Paulo(SP) UNIMAIMONIDES, 2026. Disponible en: https//www.even3.com.br/anais/II-Encuentro-Biofotonica-597461/1253617-EFFECT-OF-PRECONDITIONING-WITH-PHOTOBIOMODULATION-ON-THE-EXPRESSION-OF-MICRORNAS-RELATED-TO-CARDIAC-HYPERTROPHY-. Acceso en: 21/08/2026