ANTIMICROBIAL PHOTODYNAMIC THERAPY WITH BLUE LED IN PEDIATRIC DENTISTRY: EFFICACY OF ERYTHROSINE AND GC TRIPLAQUE IN REDUCING CARIOGENIC BIOFILM IN HIGH-RISK CHILDREN AGED 3–5 YEARS

Thainá Coube de Melo; Amanda Rafaelly Honório Mandetta; Kristianne Porta Santos Fernandes; Lara Jansiski Motta; Anna Carolina Ratto Tempestini Horliana; Raquel Agnelli Mesquita-Ferrari; Cinthya Cosme Gutierrez Duran; Sandra Kalil Bussadori

Título del Trabajo

Autores

Thainá Coube de Melo
Amanda Rafaelly Honório Mandetta
Kristianne Porta Santos Fernandes
Lara Jansiski Motta
Anna Carolina Ratto Tempestini Horliana
Raquel Agnelli Mesquita-Ferrari
Cinthya Cosme Gutierrez Duran
Sandra Kalil Bussadori

Modalidad

Protocolos de investigación

Área temática

Odontología

Fecha de publicación

14/08/2026

País de publicación

Argentina | Argentina

Idioma de la Publicación

es-419

Página del Trabajo

https://www.even3.com.br/anais/ii-encuentro-biofotonica-597461/1253549-antimicrobial-photodynamic-therapy-with-blue-led-in-pediatric-dentistry--efficacy-of-erythrosine-and-gc-triplaqu

ISBN

978-65-272-2678-9

Palabras Clave

Photodynamic therapy, dental caries, blue LED, erythrosine, dental plaque

Resumen

Dental caries is among the most prevalent chronic diseases in childhood, caused by an acidogenic oral biofilm that demineralizes dental tissues. This condition poses a clinical challenge, particularly in children aged 3 to 5 years, due to behavioral limitations and high risk of progression. Minimally invasive, safe, and well-accepted strategies have been increasingly explored, such as antimicrobial photodynamic therapy (aPDT), which combines a photosensitizing dye with a light source of compatible wavel. This study aims to assess the efficacy of aPDT with blue LED light in reducing the microbial activity of cariogenic biofilm in high caries risk children aged 3 to 5 years. This randomized clinical trial will include three parallel groups: Group 1 (control) will undergo blue LED irradiation (VALO Grand) without dye; Group 2 will receive erythrosine + blue LED; Group 3 will receive GC TriPlaque ID Gel + blue LED. The sample will comprise 71 children (about 24 per group), determined from a sample size calculation indicating 63 participants for 80% power, increased by 12% for possible losses. Screening will follow the CAMBRA protocol adapted for children, with inclusion based on high caries risk classification. Biofilm will be collected before and after intervention using a sterile auricular curette applied to the cervical buccal surface of upper second primary molars. Samples will be placed in sterile microtubes with 1 mL Brain Heart Infusion (BHI) broth and stored at 4 °C until microbiological analysis. Colony-forming unit (CFU) counts will compare microbial load pre- and post-intervention. Results are expected to support aPDT with blue LED light as a safe, effective option for controlling caries lesions in young children, providing an additional microbiological management tool in preventive pediatric dentistry.

Título del Evento: II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Ciudad del Evento: Buenos Aires
Título de las Actas del Evento: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Nombre de la Editora: Even3
Medio de Divulgación: Meio Digital

Como citar

MELO, Thainá Coube de et al.. ANTIMICROBIAL PHOTODYNAMIC THERAPY WITH BLUE LED IN PEDIATRIC DENTISTRY: EFFICACY OF ERYTHROSINE AND GC TRIPLAQUE IN REDUCING CARIOGENIC BIOFILM IN HIGH-RISK CHILDREN AGED 3–5 YEARS.. In: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud. Anais...Sao Paulo(SP) UNIMAIMONIDES, 2026. Disponible en: https//www.even3.com.br/anais/II-Encuentro-Biofotonica-597461/1253549-ANTIMICROBIAL-PHOTODYNAMIC-THERAPY-WITH-BLUE-LED-IN-PEDIATRIC-DENTISTRY--EFFICACY-OF-ERYTHROSINE-AND-GC-TRIPLAQU. Acceso en: 21/08/2026