PHOTOBIOMODULATION EFFECTS ON THE VIABILITY OF C2C12 MUSCLE CELLS EXPOSED TO DEXAMETHASONE

Alessandra Lima da Silva Martins; Tainá Caroline dos Santos Malavazzi; Rosani Tereza De Siqueira E Silva; Aline Souza Silva; Claudio Teruo Kassa; Cinthya Cosme Gutierrez Duran; Sandra Kalil Bussadori; Anna Carolina Ratto Tempestinni Horliana; Kristianne Porta Santos Fernandes; Raquel Agnelli Mesquita-Ferrari

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Trabajo

Título del Trabajo

PHOTOBIOMODULATION EFFECTS ON THE VIABILITY OF C2C12 MUSCLE CELLS EXPOSED TO DEXAMETHASONE

Autores

Alessandra Lima da Silva Martins
Tainá Caroline dos Santos Malavazzi
Rosani Tereza De Siqueira E Silva
Aline Souza Silva
Claudio Teruo Kassa
Cinthya Cosme Gutierrez Duran
Sandra Kalil Bussadori
Anna Carolina Ratto Tempestinni Horliana
Kristianne Porta Santos Fernandes
Raquel Agnelli Mesquita-Ferrari

Modalidad

Investigación

Área temática

Medicina

Fecha de publicación

14/08/2026

País de publicación

Argentina | Argentina

Idioma de la Publicación

es-419

Página del Trabajo

https://www.even3.com.br/anais/ii-encuentro-biofotonica-597461/1253211-photobiomodulation-effects-on-the-viability-of-c2c12-muscle-cells-exposed-to-dexamethasone

ISBN

978-65-272-2678-9

Palabras Clave

C2C12; dexamethasone; muscle cells; photobiomodulation

Resumen

INTRODUCTION: Skeletal muscle homeostasis relies on the balance between proliferation, differentiation, and regeneration, which can be impaired by pathological conditions or prolonged glucocorticoid exposure. Dexamethasone (DEXA), a synthetic glucocorticoid, is widely used for its anti-inflammatory and immunosuppressive properties, but chronic or high-dose administration induces muscle atrophy. Photobiomodulation (PBM) has demonstrated anti-inflammatory and regenerative effects in muscle cells. However, its ability to counteract glucocorticoid-induced cytotoxicity remains underexplored. OBJECTIVE: This study aimed to evaluate the effects of PBM on the viability of C2C12 myoblasts exposed to different concentrations of DEXA during differentiation. METHODOS: C2C12 cells were cultured in DMEM with 10% fetal bovine serum for proliferation and differentiated in DMEM with 2% horse serum. Cells were incubated with DEXA at 5, 10, or 200 µM for 24, 48, or 72 h. PBM was applied using a 780 nm laser (70 mW, 15 s, 26.25 J/cm²). Cell viability was assessed by MTT assay after 48 and 72h. Experimental groups included: control, DEXA-only, and DEXA+PBM at each concentration and time point. Data were analyzed by ANOVA followed by Tukey’s post-hoc test (p<0.05). RESULTS: DEXA induced a decrease in cell viability in a time- and dose-dependent manner, with the greatest reduction at the higher concentration (200 µM). PBM significantly increased viability in cells treated with 5 or 10 µM DEXA and partially restored viability at 200 µM. At 48 h, PBM mitigated the pronounced viability loss observed with 10 and 200 µM DEXA. At 72 h, although viability remained below control levels, PBM-treated groups consistently showed higher values than DEXA-only groups. PBM alone had no detrimental effect. CONCLUSION: PBM improved the viability of C2C12 myoblasts exposed to DEXA, particularly at intermediate concentrations, indicating its potential to mitigate glucocorticoid-associated muscle atrophy.

Título del Evento: II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Ciudad del Evento: Buenos Aires
Título de las Actas del Evento: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Nombre de la Editora: Even3
Medio de Divulgación: Meio Digital

Como citar

MARTINS, Alessandra Lima da Silva et al.. PHOTOBIOMODULATION EFFECTS ON THE VIABILITY OF C2C12 MUSCLE CELLS EXPOSED TO DEXAMETHASONE.. In: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud. Anais...Sao Paulo(SP) UNIMAIMONIDES, 2026. Disponible en: https//www.even3.com.br/anais/II-Encuentro-Biofotonica-597461/1253211-PHOTOBIOMODULATION-EFFECTS-ON-THE-VIABILITY-OF-C2C12-MUSCLE-CELLS-EXPOSED-TO-DEXAMETHASONE. Acceso en: 21/08/2026