IN VIVO EVALUATION OF 5-ALA–MEDIATED PHOTODYNAMIC THERAPY EFFECTS ON LEUKOCYTES IN A MOUSE MODEL OF ORAL SQUAMOUS CELL CARCINOMA

Maykon nascimento de Assis; Bibiana Padilha de Menezes da Silva; Tawany Gonçalves Santos; Ana Paula LIgeiro de Oliveira; RENATO ARAUJO PRATES; Maria Fernanda Setubal Destro Rodrigues

Título del Trabajo

Autores

Maykon nascimento de Assis
Bibiana Padilha de Menezes da Silva
Tawany Gonçalves Santos
Ana Paula LIgeiro de Oliveira
RENATO ARAUJO PRATES
Maria Fernanda Setubal Destro Rodrigues

Modalidad

Protocolos de investigación

Área temática

Odontología

Fecha de publicación

14/08/2026

País de publicación

Argentina | Argentina

Idioma de la Publicación

es-419

Página del Trabajo

https://www.even3.com.br/anais/ii-encuentro-biofotonica-597461/1248389-in-vivo-evaluation-of-5-alamediated-photodynamic-therapy-effects-on-leukocytes-in-a-mouse-model-of-oral-squamou

ISBN

978-65-272-2678-9

Palabras Clave

Oral squamous cell carcinoma, photodynamic therapy, tumor growth, immune response.

Resumen

Oral squamous cell carcinoma (OSCC) is an aggressive and treatment-resistant cancer, characterized by high recurrence and metastasis rates. Photodynamic therapy (PDT) using 5-aminolevulinic acid (5-ALA) has shown potential in various cancer types; however, its specific efficacy for OSCC remains insufficiently explored. PDT with 5-ALA offers a promising alternative to conventional therapies for OSCC, with the potential to enhance treatment efficacy and reduce side effects. However, little is known regarding its in vivo effectiveness in controlling tumor growth and the immune response in OSCC. Thus, this study aimed to evaluate the effects of 5-ALA–mediated PDT in a murine OSCC model. Forty male C57BL/6 mice were subcutaneously inoculated with the MOC2 cell line. Once tumors reached 100 mm³, animals were randomly assigned to four groups: Control, 5-ALA, Light (DMC Photon Laser, 660 nm, 100 mW, 6 J/cm²), and PDT (incubation with 5-ALA followed by laser irradiation with the parameters described). Tumor growth was assessed three times per week. Seven days after treatment, animals were euthanized, and blood samples were collected for hematological evaluation. Results showed no significant difference in tumor size and total leukocyte count among groups (p > 0.05). However, leukocyte profile analysis revealed relevant modulations: the PDT group exhibited a significant reduction in neutrophil percentage compared with the 5-ALA group (p < 0.01), suggesting a possible attenuation of the innate inflammatory acute response. Additionally, a significant increase in lymphocyte proportion was observed in PDT group in relation to Control (p < 0.05) and Light (p < 0.01) groups, indicating activation of the adaptive immune response. No significant differences were found in monocyte levels (p > 0.05). These findings suggest that 5-ALA–mediated PDT may act as an immunomodulator, promoting a more specific and potentially effective cellular immune response in OSCC treatment.

Título del Evento: II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Ciudad del Evento: Buenos Aires
Título de las Actas del Evento: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Nombre de la Editora: Even3
Medio de Divulgación: Meio Digital

Como citar

ASSIS, Maykon nascimento de et al.. IN VIVO EVALUATION OF 5-ALA–MEDIATED PHOTODYNAMIC THERAPY EFFECTS ON LEUKOCYTES IN A MOUSE MODEL OF ORAL SQUAMOUS CELL CARCINOMA.. In: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud. Anais...Sao Paulo(SP) UNIMAIMONIDES, 2026. Disponible en: https//www.even3.com.br/anais/II-Encuentro-Biofotonica-597461/1248389-IN-VIVO-EVALUATION-OF-5-ALAMEDIATED-PHOTODYNAMIC-THERAPY-EFFECTS-ON-LEUKOCYTES-IN-A-MOUSE-MODEL-OF-ORAL-SQUAMOU. Acceso en: 21/08/2026