EFFECT OF PHOTOBIOMODULATION ON THE CYTOTOXICITY AND CELL DEATH MECHANISMS IN C2C12 CELLS EXPOSED TO JUVENILE AND ADULT BOTHROPS ALTERNATUS VENOMS.

Daniele Campos Machado; Amanda Cabral David; María Emília García Denegri; Laura Cristina A. Leiva; Juliana Pavan Zuliani; Stella Regina Zamuner

Título del Trabajo

Autores

Daniele Campos Machado
Amanda Cabral David
María Emília García Denegri
Laura Cristina A. Leiva
Juliana Pavan Zuliani
Stella Regina Zamuner

Modalidad

Investigación

Área temática

Medicina

Fecha de publicación

14/08/2026

País de publicación

Argentina | Argentina

Idioma de la Publicación

es-419

Página del Trabajo

https://www.even3.com.br/anais/ii-encuentro-biofotonica-597461/1246861-effect-of-photobiomodulation-on-the-cytotoxicity-and-cell-death-mechanisms-in-c2c12-cells-exposed-to-juvenile-an

ISBN

978-65-272-2678-9

Palabras Clave

Keywords: Photobiomodulation (PBM), Cytotoxicity, Bothrops alternatus

Resumen

Bothrops snakes are of great medical and epidemiological importance, and their bites cause both systemic and serious local effects on their victims. It is also known that there are differences in the composition of the venoms of young and adult snakes, which can lead to differences in the evolution of clinical manifestations. Photobiomodulation (PBM) has been shown to be an effective adjuvant therapy to serum therapy to reduce the local effects induced by bothropic snake venom. This study evaluated the effect of PBM on C2C12 myoblast cells after the action of Bothrops alternatus snake venom (BaV) in its juvenile (BaJV) and adult (BaAV) stages. C2C12 cells received VBaJ or VBaA (50 µg/mL), or only culture medium (control), and were immediately treated with PBM. Treatment was performed using a low-level laser (660 nm wavelength, 4 J/cm², 100 mW, 0.33 W/cm², 40 seconds). Cytotoxicity, apoptosis, and necrosis were assessed 3 hours after venom exposure using LDH assays and flow cytometry to measure fluorescence emission for annexin V (apoptosis) and 7-AAD (necrosis). The results demonstrated that both venoms significantly increased cytotoxicity and induced apoptosis. PBM treatment reduced BaAV-induced apoptosis and necrosis but had no effect on BaJV-induced apoptosis, necrosis, or cytotoxicity. These findings demonstrate that both juvenile and adult venoms affect C2C12 cells, their effects differ in magnitude. PBM treatment shows protective effect against B. alternatus venom across different developmental stages, suggesting its therapeutical utility in mitigating venom-induced cytotoxicity.

Título del Evento: II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Ciudad del Evento: Buenos Aires
Título de las Actas del Evento: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Nombre de la Editora: Even3
Medio de Divulgación: Meio Digital

Como citar

MACHADO, Daniele Campos et al.. EFFECT OF PHOTOBIOMODULATION ON THE CYTOTOXICITY AND CELL DEATH MECHANISMS IN C2C12 CELLS EXPOSED TO JUVENILE AND ADULT BOTHROPS ALTERNATUS VENOMS... In: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud. Anais...Sao Paulo(SP) UNIMAIMONIDES, 2026. Disponible en: https//www.even3.com.br/anais/II-Encuentro-Biofotonica-597461/1246861-EFFECT-OF-PHOTOBIOMODULATION-ON-THE-CYTOTOXICITY-AND-CELL-DEATH-MECHANISMS-IN-C2C12-CELLS-EXPOSED-TO-JUVENILE-AN. Acceso en: 22/08/2026