OPTIMIZING ANTIMICROBIAL PHOTODYNAMIC THERAPY FOR CANDIDA ALBICANS BIOFILMS: MECHANISMS, BARRIERS, AND THERAPEUTIC POTENTIAL

Giovanna Fontgalland Ferreira; Claudio Teruo Kassa; Shirlene De Holanda lima; Valherya Silva Rodriguez; Ilka Tiemy Kato; Sarah Marinho Figueiredo; Mark Wainwright; Renato Prates

Título del Trabajo

Autores

Giovanna Fontgalland Ferreira
Claudio Teruo Kassa
Shirlene De Holanda lima
Valherya Silva Rodriguez
Ilka Tiemy Kato
Sarah Marinho Figueiredo
Mark Wainwright
Renato Prates

Modalidad

Protocolos de investigación

Área temática

Odontología

Fecha de publicación

14/08/2026

País de publicación

Argentina | Argentina

Idioma de la Publicación

es-419

Página del Trabajo

https://www.even3.com.br/anais/ii-encuentro-biofotonica-597461/1245796-optimizing-antimicrobial-photodynamic-therapy-for-candida-albicans-biofilms--mechanisms-barriers-and-therapeut

ISBN

978-65-272-2678-9

Palabras Clave

Biophotonics, Microbial resistance, Photosensitizer

Resumen

Candidiasis, a fungal infection primarily caused by Candida albicans, predominantly affects immunocompromised individuals, compromising mucosal surfaces, skin, and consequently, quality of life. Although conventional antifungal agents demonstrate clinical efficacy, the progressive increase in microbial resistance and the presence of biofilms reduce treatment effectiveness. These therapeutic limitations highlight the need for complementary strategies, among which antimicrobial photodynamic therapy (aPDT) stands out as a promising approach. aPDT combines light at a specific wavelength, photosensitizers, and molecular oxygen to generate reactive oxygen species (ROS) capable of inactivating microorganisms without inducing resistance. This project aims to investigate the mechanisms of action of aPDT at different stages and developmental times of C. albicans biofilms, optimizing parameters such as irradiation time and photosensitizer concentration, including Methylene Blue (MB) and Butyl Toluidine Blue O (BuTBO). Relevant barriers to therapeutic success, such as the biofilm extracellular matrix and cellular efflux of photosensitizers, will be addressed. Samples will be cultured in vitro, subjected to aPDT with two different photosensitizers under various experimental conditions, and comparatively evaluated among experimental groups regarding fungal growth, cell viability, photosensitizer uptake, and, finally, C. albicans susceptibility to macrophage interaction. It is expected that this study will demonstrate the efficacy of aPDT as an alternative or adjuvant to conventional antifungals, contributing to the prevention of microbial resistance associated with Candida albicans infections.

Título del Evento: II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Ciudad del Evento: Buenos Aires
Título de las Actas del Evento: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Nombre de la Editora: Even3
Medio de Divulgación: Meio Digital

Como citar

FERREIRA, Giovanna Fontgalland et al.. OPTIMIZING ANTIMICROBIAL PHOTODYNAMIC THERAPY FOR CANDIDA ALBICANS BIOFILMS: MECHANISMS, BARRIERS, AND THERAPEUTIC POTENTIAL.. In: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud. Anais...Sao Paulo(SP) UNIMAIMONIDES, 2026. Disponible en: https//www.even3.com.br/anais/II-Encuentro-Biofotonica-597461/1245796-OPTIMIZING-ANTIMICROBIAL-PHOTODYNAMIC-THERAPY-FOR-CANDIDA-ALBICANS-BIOFILMS--MECHANISMS-BARRIERS-AND-THERAPEUT. Acceso en: 22/08/2026