EVALUATION OF A CLINICAL PROTOCOL FOR PHOTODYNAMIC THERAPY IN THE ENDODONTIC TREATMENT OF PRIMARY TEETH – A RANDOMIZED CONTROLLED TRIAL

Iara Maria Freitas Romano; Lara Jansiski Motta; Kristianne Porta Santos Fernandes; Raquel Agnelli Mesquita-Ferrari; Ana Paula Taboada Sobral; Anna Carolina Ratto Tempestini Horliana; Cinthya Cosme Gutierrez Duran; Caroline C. B. Camargo; Júlio G. C. Batista; Sandra Kalil Bussadori

Título del Trabajo

Autores

Iara Maria Freitas Romano
Lara Jansiski Motta
Kristianne Porta Santos Fernandes
Raquel Agnelli Mesquita-Ferrari
Ana Paula Taboada Sobral
Anna Carolina Ratto Tempestini Horliana
Cinthya Cosme Gutierrez Duran
Caroline C. B. Camargo
Júlio G. C. Batista
Sandra Kalil Bussadori

Modalidad

Investigación

Área temática

Odontología

Fecha de publicación

14/08/2026

País de publicación

Argentina | Argentina

Idioma de la Publicación

es-419

Página del Trabajo

https://www.even3.com.br/anais/ii-encuentro-biofotonica-597461/1222665-evaluation-of-a-clinical-protocol-for-photodynamic-therapy-in-the-endodontic-treatment-of-primary-teeth--a-rand

ISBN

978-65-272-2678-9

Palabras Clave

Keywords: Antimicrobial photodynamic therapy; Endodontics; Primary teeth.

Resumen

ABSTRACT Introduction: Pulp necrosis in primary teeth, commonly caused by trauma or extensive caries, is difficult to manage due to anatomical complexity, thin dentinal walls, and frequent lack of child cooperation. Conventional endodontic treatment may not sufficiently eliminate intracanal microorganisms. Antimicrobial photodynamic therapy (aPDT), which combines a photosensitizing agent and a specific light wavelength, promotes reactive oxygen species formation, leading to effective microbial destruction without resistance, and is especially promising in pediatric dentistry due to its safety and noninvasive nature. Objective: To evaluate the efficacy and safety of a clinical aPDT protocol in the endodontic treatment of necrotic anterior primary teeth. Methodology: This randomized controlled clinical trial included 20 necrotic anterior primary teeth in children aged 2 to 5 years. Group 1 (G1) underwent conventional endodontic therapy: manual instrumentation, irrigation, and obturation. Group 2 (G2) received the same treatment plus aPDT with 0.005% methylene blue and red laser (660 nm, 100 mW, 90 s, 9 J) via optical fiber. Microbiological samples were collected using sterile absorbent paper points inserted into the canals, then cultured on blood agar under anaerobic conditions (37°C/72 h). CFUs were counted and analyzed using the Mann-Whitney test (α = 0.05). Preliminary Results: Both groups showed microbial reduction. The mean CFU reduction was higher in the aPDT group, although the difference between groups was not statistically significant (p = 0.661). No adverse events were observed, and all children tolerated the protocol well. Conclusion: This clinical aPDT protocol proved safe and well-tolerated. While no significant difference was observed in microbial reduction, results suggest potential benefits as an adjunct to conventional endodontic therapy. Continued follow-up and expanded samples are needed to confirm long-term outcomes.

Título del Evento: II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Ciudad del Evento: Buenos Aires
Título de las Actas del Evento: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Nombre de la Editora: Even3
Medio de Divulgación: Meio Digital

Como citar

ROMANO, Iara Maria Freitas et al.. EVALUATION OF A CLINICAL PROTOCOL FOR PHOTODYNAMIC THERAPY IN THE ENDODONTIC TREATMENT OF PRIMARY TEETH – A RANDOMIZED CONTROLLED TRIAL.. In: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud. Anais...Sao Paulo(SP) UNIMAIMONIDES, 2026. Disponible en: https//www.even3.com.br/anais/II-Encuentro-Biofotonica-597461/1222665-EVALUATION-OF-A-CLINICAL-PROTOCOL-FOR-PHOTODYNAMIC-THERAPY-IN-THE-ENDODONTIC-TREATMENT-OF-PRIMARY-TEETH--A-RAND. Acceso en: 21/08/2026