Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul

DETECTION OF ROTAVIRUS A (RVA) IN OYSTER-FARMING AND WATERS BEFORE AND AFTER PURIFICATION PROCESS IN A CITY IN THE STATE OF PARÁ

Published in 04/01/2021 - ISBN: 978-65-5941-175-7

Link All Papers
LinkSubmission
DOI
10.29327/cbv.295458  
Paper Title
DETECTION OF ROTAVIRUS A (RVA) IN OYSTER-FARMING AND WATERS BEFORE AND AFTER PURIFICATION PROCESS IN A CITY IN THE STATE OF PARÁ
Authors
  • Macyclelma Alves Albuquerque
  • Patrícia dos Santos Lobo
  • Dielle Monteiro Teixeira
  • Lívia Rodrigues Vaz
  • Isis Priscila Pereira de Souza
  • Luana da Silva Soares
  • Monica Cristina de Moraes Silva
  • Sylvia de Fátima dos Santos Guerra
Modality
Resumo
Subject area
Ambiental
Publishing Date
04/01/2021
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/cbv/295458-detection-of-rotavirus-a-(rva)-in-oyster-farming-and-waters-before-and-after-purification-process-in-a-city-in-th
ISBN
978-65-5941-175-7
Keywords
Rotavirus A, Environmental Virology, Water, Oyster.
Summary
Introduction: Gastroenteric diseases have been reported in outbreaks after ingestion of bivalve molluscs contaminated with enteric viruses, especially Rotavirus A (RVA), which are associated with high rates of morbidity and mortality on a global scale, which makes it a serious public health problem. Among the bivalve molluscs, oysters, which are considered potential sources of contamination of pathogenic agents, as they act as filtering organisms that feed on particles suspended in water. In view of this, purification is a process that consists of eliminating the infectious agents of these molluscs, providing a product with health assurance to the consumer market. Objectives: Detect RVA in oysters and waters before and after the process of purification in oyster-farming in the Northeast region of the state of Pará in 2019. Material and Methods: 19 samples of oysters, 3 waters from the cultivation site and 15 water samples from the purifier were collected during the months of June to December 2019 in the municipality of Salinas (Northeast of Pará). After the collection, the oysters were treated through the process of dissection, elution, concentration and clarification, while the waters by the process of flocculation. Viral extraction was performed using the commercial kit, then subjected to the Polymerase Chain Reaction preceded by Reverse Transcription in real time (RT-qPCR), with specific primers for the NSP3 gene (threshold 0.13) to detect RVA. Results: The 19 oyster samples and the 18 water samples analyzed showed a negative result for RVA in RT-qPCR test. Conclusion: The investigated RVA was not observed in oyster and water samples before and after the purification process. Although RVA has not been detected, it is necessary to keep track of periodic checks through research for enteric viruses so that these foods are distributed safely and with good hygienic quality for the population that consumes it. Financial Support: Evandro Chagas Institute, National Council for Scientific and Technological Development.
Title of the Event
XXXI Congresso Brasileiro de Virologia & XV Encontro de Virologia do Mercosul
Title of the Proceedings of the event
Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul
Name of the Publisher
Even3
Means of Dissemination
Meio Digital
DOI

How to cite

ALBUQUERQUE, Macyclelma Alves et al.. DETECTION OF ROTAVIRUS A (RVA) IN OYSTER-FARMING AND WATERS BEFORE AND AFTER PURIFICATION PROCESS IN A CITY IN THE STATE OF PARÁ.. In: Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul. Anais...Porto Alegre(RS) Online, 2020. Available in: https//www.even3.com.br/anais/cbv/295458-DETECTION-OF-ROTAVIRUS-A-(RVA)-IN-OYSTER-FARMING-AND-WATERS-BEFORE-AND-AFTER-PURIFICATION-PROCESS-IN-A-CITY-IN-TH. Access in: 14/06/2025

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