A PROMISING METHOD TO DECREASE THE DESICCATION-SENSITIVE BEHAVIOR OF EUGENIA ASTRINGENS (MYRTACEAE) SEEDS THROUGH IMBIBITION

Guilherme de Almeida Garcia Rodrigues; Danielle da Silva; Neusa Steiner

Paper Title

Authors

Guilherme de Almeida Garcia Rodrigues
Danielle da Silva
Neusa Steiner

Modality

Abstract

Subject area

Plant Physiology

Publishing Date

26/09/2022

Country of Publishing

Brasil

Language of Publishing

en-US

Paper Page

https://www.even3.com.br/anais/cbfv2022_registration/513877-a-promising-method-to-decrease-the-desiccation-sensitive-behavior-of-eugenia-astringens-(myrtaceae)-seeds-through

ISBN

978-65-5941-830-5

Keywords

wild species, recalcitrant, seed conservation, 1-butanol.

Summary

Eugenia astringens (Myrtaceae) is a non-domesticated endemic Brazilian species that produces desiccation-sensitive (DS) seeds, like over 36% of threatened plant species in the IUCN Red List. One of the causes of DS seed viability loss may derive from phosphatidic acid (PA) accumulation. This accumulation leads to leaky membranes, which intensifies seed leakage, and to increases in lipid peroxidation (MDA), which is also closely related to membrane disruption. We analyzed the ability of 1-butanol (1-BUT) and N-acylethanolamine (NAE), phosphatidic acid inhibitors, in improving seed germination upon desiccation. We imbibed E. astringens fresh seeds (1.02 g H2O g DW-1) for 24 h in distilled water (H2O), 1-BUT or NAE and desiccated them to 0.25 g H2O g DW-1, then analyzed their germination, electrolytic leakage (EL) and MDA. Non-desiccated seeds germinated 100%. However, while H2O- or 1-BUT-desiccated seeds kept viability around 55%, NAE-desiccated seeds presented 28% of germination, and non-imbibed (CNT) desiccated seeds decreased germination to 13%. Moreover, H2O-, NAE- and 1-BUT-desiccated seeds displayed 2.6-fold less EL than CNT-desiccated seeds at 0.25 g H2O g DW-1. In relation to MDA, H2O-desiccated seeds presented 44% less accumulation, while 1-BUT- and NAE-desiccated seeds presented 35 and 22% less accumulation than CNT-desiccated seeds, respectively. 1-BUT is known to inhibit PA formation and prevent its accumulation during unbalanced metabolism upon desiccation. Conversely, the mechanisms by which H2O maintains seed viability are still unknown, but may be related to a stimulation on protein synthesis upon imbibition, which could improve survival rate during desiccation and promote germination. Our results indicate the possibility of reducing the DS behavior of E. astringens through a previous imbibition in either H2O or 1-BUT, which increases germination capacity and reduces EL and MDA levels. This promising easy method can be used forward tropical species seed conservation strategies.

Title of the Event: XVIII Brazilian Congress of Plant Physiology and I Ibero-latinoamerican Congress of Plant Biology
City of the Event: Porto Alegre
Title of the Proceedings of the event: Annals of the Brazilian Congress of Plant Physiology and Ibero-latinamerican Congress of Plant Biology
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

RODRIGUES, Guilherme de Almeida Garcia; SILVA, Danielle da; STEINER, Neusa. A PROMISING METHOD TO DECREASE THE DESICCATION-SENSITIVE BEHAVIOR OF EUGENIA ASTRINGENS (MYRTACEAE) SEEDS THROUGH IMBIBITION.. In: Annals of the Brazilian Congress of Plant Physiology and Ibero-latinamerican Congress of Plant Biology. Anais...Porto Alegre(RS) CEPUCRS, 2022. Available in: https//www.even3.com.br/anais/cbfv2022_registration/513877-A-PROMISING-METHOD-TO-DECREASE-THE-DESICCATION-SENSITIVE-BEHAVIOR-OF-EUGENIA-ASTRINGENS-(MYRTACEAE)-SEEDS-THROUGH. Access in: 12/06/2026