Anais do segundo AgroRaízes: alternativas sustentáveis para a produção vegetal

FITNESS COSTS AND INHERITANCE OF CRY1F RESISTANCE IN OSTRINIA FURNACALIS ON BT MAIZE

Publicado em 24/07/2026 - ISBN: 978-65-272-2635-2

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Trabalho
Título do Trabalho
FITNESS COSTS AND INHERITANCE OF CRY1F RESISTANCE IN OSTRINIA FURNACALIS ON BT MAIZE
Autores
  • Anila Kanwal
  • Shahzaib Ali
  • Muhammad Bilal
  • Ayesha Aslam
  • Mehran Ali
  • Ricardo Silva de Santana
  • Paulo Ricardo de Sena Fernandes
  • Luis Oswaldo Viteri Jumbo
  • Gil Rodrigues dos Santos
  • Dalmarcia de Souza Carlos Mourão
Modalidade
Resumo
Área temática
Fitossanidade
Data de Publicação
24/07/2026
País da Publicação
Brasil
Idioma da Publicação
pt-BR
Página do Trabalho
https://www.even3.com.br/anais/agroraizes-731503/1621681-fitness-costs-and-inheritance-of-cry1f-resistance-in-ostrinia-furnacalis-on-bt-maize
ISBN
978-65-272-2635-2
Palavras-Chave
Key-words: Bt resistance, Asian corn borer, fitness costs, incomplete dominance, insect resistance management (IRM)
Resumo
The Asian corn borer, Ostrinia furnacalis, is a devastating pest of maize in Asia, causing yield losses of up to 50%. Transgenic Bt maize expressing Cry1F toxin has been widely used for its control; however, the evolution of resistance threatens the sustainability of this technology. This study investigates the fitness costs and inheritance pattern of Cry1F resistance in O. furnacalis to inform effective insect resistance management (IRM) strategies. Three strains of O. furnacalis were used: a susceptible strain (ACB-BtS), a Cry1F-resistant strain (ACB-FR), and their F1 hybrid progeny from reciprocal crosses. Fitness costs were assessed by rearing neonates on a non-Bt artificial diet under controlled conditions (25°C, 65% RH, 16:8 L:D). Key biological parameters (survivorship, development time, larval and pupal weight, growth rate, sex ratio, and fecundity) were recorded. Diet-overlay bioassays were conducted to determine LC50 values and the degree of dominance (D_ML) at a diagnostic concentration (320 ng/cm²). Data were analyzed using one-way and two-way ANOVA, followed by Tukey's HSD test. The resistant strain (ACB-FR) exhibited significant fitness costs, including a 38.5% reduction in egg-to-adult survivorship, a 62% reduction in fecundity (273.8 vs. 438.9 eggs/female), and significantly lower larval and pupal weights compared to the susceptible strain. The LC50 of ACB-FR was 287.27 ng/cm², a 96.17-fold resistance ratio compared to ACB-BtS (2.99 ng/cm²). The F1 hybrids showed high resistance levels (RR: 61-63), and the degree of dominance (D_ML ˜ 0.6–0.92) confirmed that resistance is autosomal and incompletely dominant. Cry1F resistance in O. furnacalis is associated with substantial fitness costs and is inherited as an incompletely dominant, autosomal trait. These findings support the use of pyramided Bt toxins (e.g., Cry1F + Vip3Aa) and high-dose/refuge strategies to delay resistance evolution and sustain Bt maize efficacy in Asia. Funding Sources: This work was sponsored by the Natural Science Foundation of Henan and the Special Fund for Independent Innovation of Henan Academy of Agricultural Sciences. Key Laboratory of Insect Pests, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, China.
Título do Evento
II Agroraízes: Alternativas Sustentáveis para a Produção Vegetal
Cidade do Evento
Gurupi
Título dos Anais do Evento
Anais do segundo AgroRaízes: alternativas sustentáveis para a produção vegetal
Nome da Editora
Even3
Meio de Divulgação
Meio Digital

Como citar

KANWAL, Anila et al.. FITNESS COSTS AND INHERITANCE OF CRY1F RESISTANCE IN OSTRINIA FURNACALIS ON BT MAIZE.. In: Anais do segundo AgroRaízes: alternativas sustentáveis para a produção vegetal. Anais...Gurupi(TO) UFT, 2026. Disponível em: https//www.even3.com.br/anais/agroraizes-731503/1621681-FITNESS-COSTS-AND-INHERITANCE-OF-CRY1F-RESISTANCE-IN-OSTRINIA-FURNACALIS-ON-BT-MAIZE. Acesso em: 06/08/2026

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