PARTIAL WOLBACHIA MULTILOCUS SEQUENCING PROFILE BASED ON FBPA, FTSZ AND GATB LOCI AND DETECTION OF NOVEL BACTERIAL HAPLOTYPES IN DIROFILARIA IMMITIS ADULTS FROM RIO DE JANEIRO, BRAZIL

Daniel Antônio Braga Lee; Bruno Alberigi; Jovêncio Mateus Sada; João Vitor dos Santos Alves da Silva; Flavya Mendes de Almeida; Elan Cardozo Paes de Almeida; Nathália da Conceição Lima; Rodrigo Brum; Marcos Rogério André; Norma Labarthe; Fábio Scott

Título do Trabalho

Autores

Daniel Antônio Braga Lee
Bruno Alberigi
Jovêncio Mateus Sada
João Vitor dos Santos Alves da Silva
Flavya Mendes de Almeida
Elan Cardozo Paes de Almeida
Nathália da Conceição Lima
Rodrigo Brum
Marcos Rogério André
Norma Labarthe
Fábio Scott

Modalidade

Resumo

Área temática

Helmintologia

Data de Publicação

24/09/2026

País da Publicação

Brasil

Idioma da Publicação

pt-BR

Página do Trabalho

https://www.even3.com.br/anais/23-congresso-brasileiro-de-parasitologia-veterinaria-673729/1565527-partial-wolbachia-multilocus-sequencing-profile-based-on-fbpa-ftsz-and-gatb-loci-and-detection-of-novel-bacteri

ISBN

978-65-272-2733-5

Palavras-Chave

Haplotypes, nematodes, endosymbiont

Resumo

Wolbachia is a genus of Gram-negative intracellular symbiotic bacteria that infect a wide range of arthropods and nematodes, including Dirofilaria immitis. The present study aimed to molecularly detect and characterize Wolbachia haplotypes in adult filariids of D. immitis obtained from dogs through surgical procedures or necropsy performed at a private veterinary clinic and at the two Federal Universities of the state, respectively. DNA was extracted from 81 adult worms (78 females and 3 males) and subjected to PCR targeting the endogenous filarial cox-1 gene. All samples tested positive (81/81; 100%) and were subsequently subjected to PCR assays targeting five MLST loci (gatB, coxA, hcpA, ftsZ, and fbpA) and additional molecular markers (16S rRNA, groEL, gyrA, and dnaA). Allelic comparisons against the Wolbachia MLST database demonstrated that the obtained sequences were most closely related to gatB allele 192 and fbpA allele 247, both previously described from Wolbachia supergroup C associated with Onchocerca cervipedis in Canada, as well as to ftsZ allele 152, previously reported for the Wolbachia MP3 strain infecting D. immitis in the United States (USA). Complete sequence type (ST) assignment was not possible because amplification of the coxA and hcpA loci failed. In addition to the MLST loci, positive amplification and high-quality sequences were also obtained for the 16S rRNA and groEL genic regions. Phylogenetic analyses (based on five genic regions: 16S rRNA, fbpA, ftsZ, gatB, and groEL) clustered the sequences obtained in the present study within supergroup D, a clade comprising Wolbachia spp. associated with nematodes. Genotypic analyses revealed novel haplotypes for the 16S rRNA, fbpA, and gatB loci. This study represents the first report of Wolbachia haplotypes associated with D. immitis in Brazil. Three 16S rRNA haplotypes were identified, including two not yet described Brazilian haplotypes and one shared with isolates from Italy, Myanmar, Portugal, Puerto Rico, Russia, South Korea, and USA. Six fbpA haplotypes were detected: five have been described only from Brazilian samples and one was shared with isolates from France and USA. Two gatB haplotypes were identified, including one unique Brazilian haplotype and another shared with sequences from France and USA. The genotypic analyses based on the ftsZ and groEL loci revealed a single haplotype shared between Brazilian sequences and sequences detected in Cuba, France, Italy, Thailand and USA. Further studies should investigate the genetic diversity and distribution of Wolbachia haplotypes in Brazil and their role in the pathogenesis of canine dirofilariasis.

Título do Evento: 23º Congresso Brasileiro de Parasitologia Veterinária
Cidade do Evento: Belo Horizonte
Título dos Anais do Evento: Anais do XXIII Congresso Brasileiro de Parasitologia Veterinária
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

LEE, Daniel Antônio Braga et al.. PARTIAL WOLBACHIA MULTILOCUS SEQUENCING PROFILE BASED ON FBPA, FTSZ AND GATB LOCI AND DETECTION OF NOVEL BACTERIAL HAPLOTYPES IN DIROFILARIA IMMITIS ADULTS FROM RIO DE JANEIRO, BRAZIL.. In: Anais do XXIII Congresso Brasileiro de Parasitologia Veterinária. Anais...Belo Horizonte(MG) Centro de Atividades Didáticas I (CAD 1) da Universidade Federal de Minas Gerais (UFMG), 2026. Disponível em: https//www.even3.com.br/anais/23-congresso-brasileiro-de-parasitologia-veterinaria-673729/1565527-PARTIAL-WOLBACHIA-MULTILOCUS-SEQUENCING-PROFILE-BASED-ON-FBPA-FTSZ-AND-GATB-LOCI-AND-DETECTION-OF-NOVEL-BACTERI. Acesso em: 01/11/2026