EVALUATION OF PROTECTION AGAINST TOXOPLASMA GONDII FORMATION TISSUE CYSTS IN BRAIN MICE VACCINATED WITH DNA VACCINE (ROP18 AND SAG1)

João Pedro Sasse; Ana Flavia Minutti; Luiz Daniel de Barros; Beatriz de Souza Lima Nino; Fernando de Souza Rodrigues; ANA CAROLINA CAVALLIERI; Mateus Siqueira Pyles; Caroline Perucelo Constante; Maria Júlia Papin  Filadelfo; Joao Luis Garcia

Título do Trabalho

Autores

João Pedro Sasse
Ana Flavia Minutti
Luiz Daniel de Barros
Beatriz de Souza Lima Nino
Fernando de Souza Rodrigues
ANA CAROLINA CAVALLIERI
Mateus Siqueira Pyles
Caroline Perucelo Constante
Maria Júlia Papin Filadelfo
Joao Luis Garcia

Modalidade

Resumo

Área temática

Protozoologia e patógenos transmitidos por vetores

Data de Publicação

24/09/2026

País da Publicação

Brasil

Idioma da Publicação

pt-BR

Página do Trabalho

https://www.even3.com.br/anais/23-congresso-brasileiro-de-parasitologia-veterinaria-673729/1564032-evaluation-of-protection-against-toxoplasma-gondii-formation-tissue-cysts-in-brain-mice-vaccinated-with-dna-vacc

ISBN

978-65-272-2733-5

Palavras-Chave

DNA vectors, transfection, VERO cell, proteins.

Resumo

Toxoplasma gondii is a protozoan that causes subclinical infection, as well as spontaneous abortions in some animal species and humans. Once the alternatives to control toxoplasmosis is a development of vaccines. This study aimed to evaluate the humoral immune response and protection against tissue cysts in mice immunized with DNA vaccines with ROP18 and SAG1. The proteins were cloned, and transfections were performed in VERO cells to test cell transformation, which was observed by Polymerase Chain Reaction using cell lysate, protein, and vector primers. Mice were divided into 4 groups with nine each group (G1 pcDNASAG1, G2 pcDNAROP18, G3 pcDNA, and G4 pdDNASAG1+pcDNAROP18). The mice received 35 µg of plasmids at each dose performed between days 0, 21, and 42 via the intramuscular route, and the challenge was performed on day 87 with 25 brain cysts of the ME49 strain of T. gondii given orally. The animals were euthanized on day 140, and their brains were collected for subsequent brain cyst counting. The survival rate between 44% in group vaccinated with both protein and 88% in group vaccinated with SAG1 mice. The lowest survival rate was observed in the group vaccinated with both proteins. Regarding the quantification of brain cysts, the animals in the vaccinated groups showed a statistically significant decrease in cyst counts compared to the control group, particularly in the group vaccinated with the SAG1 protein (p < 0.05). Therefore, we concluded that our proteins offer partial protection against T. gondii infection and could be considered good vaccine candidates.

Título do Evento: 23º Congresso Brasileiro de Parasitologia Veterinária
Cidade do Evento: Belo Horizonte
Título dos Anais do Evento: Anais do XXIII Congresso Brasileiro de Parasitologia Veterinária
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

SASSE, João Pedro et al.. EVALUATION OF PROTECTION AGAINST TOXOPLASMA GONDII FORMATION TISSUE CYSTS IN BRAIN MICE VACCINATED WITH DNA VACCINE (ROP18 AND SAG1).. In: Anais do XXIII Congresso Brasileiro de Parasitologia Veterinária. Anais...Belo Horizonte(MG) Centro de Atividades Didáticas I (CAD 1) da Universidade Federal de Minas Gerais (UFMG), 2026. Disponível em: https//www.even3.com.br/anais/23-congresso-brasileiro-de-parasitologia-veterinaria-673729/1564032-EVALUATION-OF-PROTECTION-AGAINST-TOXOPLASMA-GONDII-FORMATION-TISSUE-CYSTS-IN-BRAIN-MICE-VACCINATED-WITH-DNA-VACC. Acesso em: 01/11/2026