Anais do XXIII Congresso Brasileiro de Parasitologia Veterinária

MOLECULAR INVESTIGATION OF BACTERIAL AGENTS IN CHIGGER MITES FROM MULTIPLE HOSTS ACROSS DIFFERENT REGIONS OF BRAZIL

Publicado em 24/09/2026 - ISBN: 978-65-272-2733-5

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Trabalho
Título do Trabalho
MOLECULAR INVESTIGATION OF BACTERIAL AGENTS IN CHIGGER MITES FROM MULTIPLE HOSTS ACROSS DIFFERENT REGIONS OF BRAZIL
Autores
  • Ana Cláudia Calchi
  • Isabella Pereira Pesenato
  • Maria Vitória
  • vithoria alves barbosa
  • Ester Nascimento da Costa
  • Murillo Castelo Branco Monho
  • Giovanna Rafaeli Rezende
  • Pedro Henrique Alves De Freitas
  • RICARDO BASSINI SILVA
  • Fernando de Castro Jacinavicius
Modalidade
Resumo
Área temática
Protozoologia e patógenos transmitidos por vetores
Data de Publicação
24/09/2026
País da Publicação
Brasil
Idioma da Publicação
pt-BR
Página do Trabalho
https://www.even3.com.br/anais/23-congresso-brasileiro-de-parasitologia-veterinaria-673729/1561404-molecular-investigation-of-bacterial-agents-in-chigger-mites-from-multiple-hosts-across-different-regions-of-bra
ISBN
978-65-272-2733-5
Palavras-Chave
Trombiculidae, Bartonella spp., Ehrlichiaceae, Borrelia spp., Orientia spp.
Resumo
Chigger mites are a group of mites comprising three families (Trombiculidae, Leeuwenhoekiidae, and Walchiidae) whose larval stage is parasitic. They are of medical and veterinary importance because they parasitize animals and accidentally humans, potentially causing dermatitis and acting as vectors of Orientia spp., zoonotic bacteria responsible for scrub typhus. In addition, DNA from Rickettsia spp., Anaplasma phagocytophilum, Ehrlichia sp., Bartonella spp., Borrelia spp., Coxiella burnetii, and Hepatozoon spp. has been detected in these mites; however, experimental studies confirming their vector competence are still lacking. The present study aimed to detect Bartonella spp., Erlichiaceae agents, Borrelia spp., and Orientia spp. in chigger mites deposited in the Acarological Collection of the Butantan Institute (IBSP) and collected from different vertebrate hosts (orders Rodentia, Carnivora, Chiroptera, Didelphimorphia, Galliformes, and Passeriformes) across fifteen Brazilian states. For this purpose, DNA from 248 mites (genera Apolonia, Blankaartia, Eutrombicula, Herpetacarus, Microtrombicula, Pseudoschoengastia and Quadraseta) was individually extracted. The presence of amplifiable DNA in all samples was confirmed by PCR targeting the endogenous 18S rRNA gene. Samples were screened for Bartonella spp. (cPCR targeting the nuoG gene), Ehrlichiaceae (cPCR targeting the 16S rRNA gene), Borrelia spp. (qPCR targeting the 16S rRNA gene), and Orientia spp. (qPCR targeting the 16S rRNA gene), and all samples tested negative for the investigated agents. The lack of microorganisms detection may be associated with low parasitic loads, the limited sensitivity of the detection methods used for this type of sample, or the absence of infection in the analyzed mites. Further studies with larger sample sizes and more sensitive techniques, such as third-generation molecular approaches (e.g., digital PCR) and next-generation sequencing, are needed to better understand the circulation of these agents in these ectoparasites.
Título do Evento
23º Congresso Brasileiro de Parasitologia Veterinária
Cidade do Evento
Belo Horizonte
Título dos Anais do Evento
Anais do XXIII Congresso Brasileiro de Parasitologia Veterinária
Nome da Editora
Even3
Meio de Divulgação
Meio Digital

Como citar

CALCHI, Ana Cláudia et al.. MOLECULAR INVESTIGATION OF BACTERIAL AGENTS IN CHIGGER MITES FROM MULTIPLE HOSTS ACROSS DIFFERENT REGIONS OF BRAZIL.. In: Anais do XXIII Congresso Brasileiro de Parasitologia Veterinária. Anais...Belo Horizonte(MG) Centro de Atividades Didáticas I (CAD 1) da Universidade Federal de Minas Gerais (UFMG), 2026. Disponível em: https//www.even3.com.br/anais/23-congresso-brasileiro-de-parasitologia-veterinaria-673729/1561404-MOLECULAR-INVESTIGATION-OF-BACTERIAL-AGENTS-IN-CHIGGER-MITES-FROM-MULTIPLE-HOSTS-ACROSS-DIFFERENT-REGIONS-OF-BRA. Acesso em: 01/11/2026

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