IS THERE CIRCULATION OF ANAPLASMATACEAE AGENTS IN WHITE-LIPPED PECCARIES (TAYASSU PECARI) FROM THE CERRADO BIOME, MIDWESTERN BRAZIL?

Nicolas Jalowitzki de Lima; Beatriz Silva Gonzaga; Raphaela Bueno Mendes Bittencourt; Ennya Rafaella Neves Cardoso; Mariana Camili da Silva; Mariana Avelar Tavares; Rodrigo Araújo Camêlo; Raphael Ribeiro Francisco; Gonzalo Barquero; Felipe da Silva Krawczak

Título do Trabalho

Autores

Nicolas Jalowitzki de Lima
Beatriz Silva Gonzaga
Raphaela Bueno Mendes Bittencourt
Ennya Rafaella Neves Cardoso
Mariana Camili da Silva
Mariana Avelar Tavares
Rodrigo Araújo Camêlo
Raphael Ribeiro Francisco
Gonzalo Barquero
Felipe da Silva Krawczak

Modalidade

Resumo

Área temática

Protozoologia e patógenos transmitidos por vetores

Data de Publicação

24/09/2026

País da Publicação

Brasil

Idioma da Publicação

pt-BR

Página do Trabalho

https://www.even3.com.br/anais/23-congresso-brasileiro-de-parasitologia-veterinaria-673729/1547782-is-there-circulation-of-anaplasmataceae-agents-in-white-lipped-peccaries-(tayassu-pecari)-from-the-cerrado-biome

ISBN

978-65-272-2733-5

Palavras-Chave

qPCR; wildlife reservoirs; One Health; enzootic cycles; molecular epidemiology

Resumo

Anaplasmataceae comprises obligate intracellular bacteria transmitted by hematophagous arthropods, including the genera Anaplasma and Ehrlichia, which are relevant to veterinary medicine and public health. In Brazil, Anaplasma marginale causes economic losses in livestock production, while Anaplasma platys and Ehrlichia canis are mainly associated with infections in dogs. Recent studies have also demonstrated the circulation of Anaplasmataceae agents in wildlife, including cervids, birds, and primates, as well as the occurrence of novel, still uncharacterized genotypes. In suiforms, the molecular detection of Anaplasma and Ehrlichia has already been described in wild boars (Sus scrofa) and Tayassu pecari, suggesting the potential participation of these animals in transmission dynamics among wildlife, domestic animals, and humans, particularly in regions with intense anthropogenic-environmental interfaces. Thus, this study aimed to evaluate the prevalence of Anaplasmataceae agents in a population of T. pecari from natural and rural areas surrounding Emas National Park, using the 23S rRNA region (~169 bp) as the molecular target. Sampling was performed in 2025 and included animals introduced into captivity until 2022. Animal capture and handling were conducted in partnership with the Tropical Sustainability Institute (TSI) as part of a population management program for T. pecari. Whole blood samples were collected in EDTA tubes and stored at -20 °C until processing. DNA extraction was performed using an adapted manual magnetic bead protocol, and molecular analyses were conducted by qPCR using a hydrolysis probe system. Each animal was also inspected for the presence of arthropod vectors, and 20 animals were chemically restrained to allow a more detailed examination of lateral body regions for five minutes. Results were analyzed using confidence intervals and associations with sex, age group (young or adult), and animal origin (captive-born or wild-born). Associations were evaluated using Fisher’s exact test and binomial confidence interval estimates. Of the 280 samples analyzed, 195 were positive for 23S rRNA, corresponding to a prevalence of 69.6% (95% CI: 63.9–75.0%). Significant associations were observed between positivity and sex (p=0.007), age (p<0.001), and origin (p<0.001), with higher positivity in males, adults, and wild-born animals. Five samples were sequenced, and preliminary BLAST analysis revealed 99.1% similarity to Anaplasma previously detected in xenarthrans (Accession number PX496885.1). No arthropod vectors were found parasitizing the animals. These findings suggest that Tayassu pecari may participate in the maintenance of Anaplasma in wildlife transmission cycles.

Título do Evento: 23º Congresso Brasileiro de Parasitologia Veterinária
Cidade do Evento: Belo Horizonte
Título dos Anais do Evento: Anais do XXIII Congresso Brasileiro de Parasitologia Veterinária
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

LIMA, Nicolas Jalowitzki de et al.. IS THERE CIRCULATION OF ANAPLASMATACEAE AGENTS IN WHITE-LIPPED PECCARIES (TAYASSU PECARI) FROM THE CERRADO BIOME, MIDWESTERN BRAZIL?.. In: Anais do XXIII Congresso Brasileiro de Parasitologia Veterinária. Anais...Belo Horizonte(MG) Centro de Atividades Didáticas I (CAD 1) da Universidade Federal de Minas Gerais (UFMG), 2026. Disponível em: https//www.even3.com.br/anais/23-congresso-brasileiro-de-parasitologia-veterinaria-673729/1547782-IS-THERE-CIRCULATION-OF-ANAPLASMATACEAE-AGENTS-IN-WHITE-LIPPED-PECCARIES-(TAYASSU-PECARI)-FROM-THE-CERRADO-BIOME. Acesso em: 16/10/2026