OVICIDAL AND LARVICIDAL ACTIVITY OF DISOPHENOL IN VITRO AGAINST EQUINE GASTROINTESTINAL NEMATODES.

Leticia Dutra Cirne; CLÓVIS DE PAULA SANTOS

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Trabalho

Título do Trabalho

OVICIDAL AND LARVICIDAL ACTIVITY OF DISOPHENOL IN VITRO AGAINST EQUINE GASTROINTESTINAL NEMATODES.

Autores

Leticia Dutra Cirne
CLÓVIS DE PAULA SANTOS

Modalidade

Resumo

Área temática

Helmintologia

Data de Publicação

24/09/2026

País da Publicação

Brasil

Idioma da Publicação

pt-BR

Página do Trabalho

https://www.even3.com.br/anais/23-congresso-brasileiro-de-parasitologia-veterinaria-673729/1543216-ovicidal-and-larvicidal-activity-of-disophenol-in-vitro-against-equine-gastrointestinal-nematodes

ISBN

978-65-272-2733-5

Palavras-Chave

Anthelmintic, dewormer, nematode control.

Resumo

The challenges in controlling gastrointestinal parasites in horses, exacerbated by the diminishing efficacy of commercial anthelmintics, necessitate research into antiparasitic molecules not yet labeled for equine use. This study evaluated the in vitro ovicidal and larvicidal activity of disophenol against equine gastrointestinal nematodes. Eggs were recovered from fecal samples of naturally infected horses at the Animal Research Support Unit of the State University of Northern Rio de Janeiro (UENF). For the egg hatching test (EHT), feces were homogenized and filtered through a sequence of granulometric sieves (24 to 400 mesh); the retained eggs were then purified via centrifugation at 1600g for three minutes. In 24-well plates, 10 µL of an egg suspension (~100 eggs) was combined with 200 µL of disophenol (Rumivac 30 Injectable) at concentrations ranging from 0.03 to 300 mg, using distilled water as a control. Following incubation at room temperature (22–28°C), plates were monitored at 24 and 48 hours for photomicrography and quantification. The assay, performed in triplicate, revealed total hatching inhibition at 30 and 300 mg, while the 3 mg concentration yielded hatching rates below 2%. Conversely, concentrations of 0.3 and 0.03 mg resulted in over 90% hatching. Notably, all larvae in the treated groups died within 24 hours, exhibiting significant morphological damage, whereas control larvae remained viable throughout the 48-hour period. These findings demonstrate the potent activity of disophenol against equine nematodes, suggesting its potential integration into equine parasite control strategies.

Título do Evento: 23º Congresso Brasileiro de Parasitologia Veterinária
Cidade do Evento: Belo Horizonte
Título dos Anais do Evento: Anais do XXIII Congresso Brasileiro de Parasitologia Veterinária
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

CIRNE, Leticia Dutra; SANTOS, CLÓVIS DE PAULA. OVICIDAL AND LARVICIDAL ACTIVITY OF DISOPHENOL IN VITRO AGAINST EQUINE GASTROINTESTINAL NEMATODES... In: Anais do XXIII Congresso Brasileiro de Parasitologia Veterinária. Anais...Belo Horizonte(MG) Centro de Atividades Didáticas I (CAD 1) da Universidade Federal de Minas Gerais (UFMG), 2026. Disponível em: https//www.even3.com.br/anais/23-congresso-brasileiro-de-parasitologia-veterinaria-673729/1543216-OVICIDAL-AND-LARVICIDAL-ACTIVITY-OF-DISOPHENOL-IN-VITRO-AGAINST-EQUINE-GASTROINTESTINAL-NEMATODES. Acesso em: 13/10/2026