Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul

GENETIC DIVERSITY AMONG PCV3 SHOWED TWO MAIN LINEAGES IN BRAZILIAN STRAINS AND TWO DIFFERENT STRAINS INFECTING THE SAME ANIMAL

Published in 04/01/2021 - ISBN: 978-65-5941-175-7

Link All Papers
LinkSubmission
DOI
10.29327/cbv.296899  
Paper Title
GENETIC DIVERSITY AMONG PCV3 SHOWED TWO MAIN LINEAGES IN BRAZILIAN STRAINS AND TWO DIFFERENT STRAINS INFECTING THE SAME ANIMAL
Authors
  • Viviane Sisdelli Assao
  • Marcus Rebouças Santos
  • Laura Morais Nascimento Silva
  • Nívia Carolina Lopes Rosado
  • Larissa Lana de Paula Leber
  • Lidiany Lopes Gomes
  • Junio Cesar Santos
  • Pedro Marcus Pereira Vidigal
  • Abelardo Silva Júnior
Modality
Resumo
Subject area
Veterinária
Publishing Date
04/01/2021
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/cbv/296899-genetic-diversity-among-pcv3-showed-two-main-lineages-in-brazilian-strains-and-two-different-strains-infecting-th
ISBN
978-65-5941-175-7
Keywords
Porcine circovirus, genetic diversity, swine
Summary
Porcine circovirus 3 (PCV3) has drawn the attention of the swine industry since it was identified in 2016 through a metagenomics approach in swine with different clinical signs. Since the first report, researchers from many countries around the world have been reporting the detection of PCV3. In Brazil, a retrospective study detected PCV3 DNA in swine samples from 1967 and is the oldest PCV3 partial capsid sequence described until now. About the PCV3 genetic organization, it is known that PCV3 has an ambisense genome organization, with 1999-2001 nucleotides, and encodes three open reading frames (ORF). ORF2 encodes an amino acid capsid which is related to virulence. This work aimed to identify PCV3 from Brazilian samples and sequence the region of the capsid protein. We analyzed 261 swine samples from swine of different life stages. These samples were collected in 2019 from 19 swine farms located in Minas Gerais State, which is an important swine producer in Brazil. Total DNA was extracted from samples using Wizard SV Genomic DNA Purification System. For PCV3 detecting a quantitative real-time PCR (qPCR). Positive samples were submitted to Sanger sequencing and the data were analyzed using CLC Genomics Workbench version 8.5.4. Sequences were further clustered using the cd-hit-est tool of CD-HIT version 4.7 to remove redundancy, i.e. identical sequences identified in different samples. The ORF2 sequences were aligned by MAFFT version 7.307. Our results indicated that 39.85% (104/261) of the animals tested were PCV3 positive. Among the PCV3 positive samples, 17 PCV3 positive samples had the ORF2 sequenced. The sequences of ORF2 were clustered into four non-redundant sequences (UFV01/BR/MG/2019, UFV02/BR/MG/2019, UFV03/BR/MG/2019, and UFV04/BR/MG/2019), and were deposited in the GenBank database (MT497513, MT497514, MT497515, and MT497516). Analysis of the genome of the sequences showed an identity of nucleotides (98.98 to 100%) and amino acids (97.66 to 100%) among different PCV3 strains. In the phylogenetic analysis, all Brazilian strains were classified in the monophyletic clade of the PCV3a genotype. We also observed amino acid mutations in PCV3 cap protein that suggests that different PCV3 strains are circulating in Brazilian swine herds. We performed a phylogenetic analysis with the 17 sequences identified in this study and the PCV3 Brazilian cap sequence previously deposited into GenBank. Our results demonstrate that PCV3 Brazilian strains can be arranged into four different clusters and it could be divided into at least two main lineages. In this work, two strains (UFV/01/MG/BR/2019 and UFV/02/MG/BR/2019) were obtained from different tissue samples (lymph node and intestine) of the same animal, which presented clinical signs of wasting. This result confirms that more than one PCV3 strain might be infecting the same animal. This study identified PCV3 in swine farms in Minas Gerais State, sequenced 17 PCV3 capsid proteins, and demonstrated the existence of genetic diversity among the PCV3 strains circulating in Brazilian swine herds.
Title of the Event
XXXI Congresso Brasileiro de Virologia & XV Encontro de Virologia do Mercosul
Title of the Proceedings of the event
Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul
Name of the Publisher
Even3
Means of Dissemination
Meio Digital
DOI

How to cite

ASSAO, Viviane Sisdelli et al.. GENETIC DIVERSITY AMONG PCV3 SHOWED TWO MAIN LINEAGES IN BRAZILIAN STRAINS AND TWO DIFFERENT STRAINS INFECTING THE SAME ANIMAL.. In: Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul. Anais...Porto Alegre(RS) Online, 2020. Available in: https//www.even3.com.br/anais/cbv/296899-GENETIC-DIVERSITY-AMONG-PCV3-SHOWED-TWO-MAIN-LINEAGES-IN-BRAZILIAN-STRAINS-AND-TWO-DIFFERENT-STRAINS-INFECTING-TH. Access in: 13/06/2025

Paper

Even3 Publicacoes