MYCELIAL DEVELOPMENT RESPONSE OF TRICHODERMA CULTIVATED IN CULTURE MEDIUM WITH DIFFERENT CONCENTRATIONS OF TREATED DAIRY EFFLUENT

Ana Carolina Murad Lima; AMANDA ALINE DA SILVA GAZITO; Fabrício Rossi

Paper Title

Authors

Ana Carolina Murad Lima
AMANDA ALINE DA SILVA GAZITO
Fabrício Rossi

Modality

Abstract

Subject area

Fungal biology in extreme environments

Publishing Date

15/05/2019

Country of Publishing

Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/isfus/160476-mycelial-development-response-of-trichoderma-cultivated-in-culture-medium-with-different-concentrations-of-treate

ISBN

978-85-5722-210-6

Keywords

filamentous fungi, stress, salinity, dairy effluent, mycelial growth

Summary

Filamentous fungi of the Trichoderma genus are found in a wide range of ecosystems, and may favor cultures in resistance to pathogens, physiological responses and resistance to saline environments. The treated dairy effluent has as main characteristic of high saline and nitrogen concentration, besides lower concentrations of other nutrients, which makes it interesting for reuse in agriculture. Three Trichoderma species were used in this study: Trichoderma atroviride, Trichoderma asperellum and Trichoderma harzianum. These strains were evaluated for their resistance to saline environments in in vitro tests. In order to do so, the strains were cultured in potato agar dextrose (BDA) medium with increasing concentrations of treated dairy effluent, diluted in distilled water, which were: 0; 25; 50; 75 and 100%. These concentrations generated mean salinity levels of 0.95; 2.24; 3.54; 4.79 and 6.03 dS m-1. The experimental design was performed in duplicate, in a completely randomized factorial (3x5), with three repetitions, each one consisting of a Petri dish. Petri dishes and culture media of each treatment were autoclaved for 20 minutes at 1 atm. The inoculum of the Trichoderma strains on the plates was made from 5 mm disks of BDA culture medium containing the active fungus transferred in a sterile way to the Petri dishes. The plates were maintained in a BOD incubator at 27 °C and 12 hours photoperiod. The mycelial growth was evaluated every 24 hours after the inoculation, measuring the colony diameter with a digital caliper. Measurements were made for three days, until the first Petri dish was completely taken by the fungal colony. Then the mycelial growth was calculated by the formula of the mycelial growth rate index (MGRI): MGRI = S [(D-Db) / N], where D is the mean diameter of the colony on day N, Db is the diameter of the colony from the previous day and N is the number of days after inoculation. The results of the statistical analysis showed that the Trichoderma asperellum and Trichoderma harzianum strains had no significant difference between them in the different effluent concentrations tested. Trichoderma atroviride presented lower MGRI in almost all the effluent concentrations, except of the treatment 100% effluent, in which there was no statistical difference between the studied strains. The tree strains had linear decrease of the MGRI as the effluent concentration in culture medium increased, presenting (R)² correlation coefficients upper to 89%. The results obtained show that the studied strains of Trichoderma are capable of developing in environment salinized by treated dairy effluent. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.

Title of the Event: III International Symposium on Fungal Stress – ISFUS
City of the Event: São José dos Campos
Title of the Proceedings of the event: Annals of the International Symposium on Fungal Stress – ISFUS
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

LIMA, Ana Carolina Murad; GAZITO, AMANDA ALINE DA SILVA; ROSSI, Fabrício. MYCELIAL DEVELOPMENT RESPONSE OF TRICHODERMA CULTIVATED IN CULTURE MEDIUM WITH DIFFERENT CONCENTRATIONS OF TREATED DAIRY EFFLUENT.. In: Annals of the International Symposium on Fungal Stress – ISFUS. Anais...São José dos Campos(SP) Hotel Nacional Inn, 2019. Available in: https//www.even3.com.br/anais/ISFUS/160476-MYCELIAL-DEVELOPMENT-RESPONSE-OF-TRICHODERMA-CULTIVATED-IN-CULTURE-MEDIUM-WITH-DIFFERENT-CONCENTRATIONS-OF-TREATE. Access in: 08/05/2025